Evaluation of the effect of oleuropein on alveolar bone loss, inflammation, and apoptosis in experimental periodontitis
Yazarlar (5)
Doç. Dr. Mehmet Murat TAŞKAN Tokat Gaziosmanpaşa Üniversitesi, Türkiye
Prof. Dr. Hatice BALCI YÜCE Tokat Gaziosmanpaşa Üniversitesi, Türkiye
Doç. Dr. Özkan KARATAŞ Tokat Gaziosmanpaşa Üniversitesi, Türkiye
Prof. Dr. Fikret GEVREK Tokat Gaziosmanpaşa Üniversitesi, Türkiye
Prof. Dr. Hülya Toker Sağlık Bilimleri Üniversitesi, Türkiye
| Makale Türü | Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | JOURNAL OF PERIODONTAL RESEARCH (Q1) | ||
| Dergi ISSN | 0022-3484 Dergi Bilgileri (2019) | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 12-2019 |
| Cilt / Sayı / Sayfa | 54 / 6 / 624–632 | DOI | 10.1111/jre.12662 |
| Makale Linki | https://onlinelibrary.wiley.com/doi/abs/10.1111/jre.12662 | ||
| UAK Araştırma Alanları |
Periodontoloji
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| Özet |
| The present study aimed to evaluate the effects of oleuropein on ligature-induced alveolar bone loss. In this respect, osteoblastic activity, osteoclastic activity, inflammatory markers, and apoptosis were evaluated. Oleuropein is a flavonoid, which has potent anti-inflammatory and bone-protective effects. Thirty-two Wistar rats were divided into four experimental groups as following: control (C, n = 8) group; periodontitis (P, n = 8) group; periodontitis and low-dose oleuropein group (12 mg/kg/day oleuropein, LDO group, n = 8); and periodontitis and high-dose oleuropein group (24 mg/kg/day oleuropein, HDO group, n = 8). Periodontitis was induced via ligatures. Study period was 14 days, and animals were sacrificed at end of this period. Mandibles were examined via a stereomicroscope and underwent histological procedures. Osteoblast, tartrate-resistant acid phosphatase (TRAP)-positive osteoclast, and inflammatory cell counts were determined in hematoxylin-eosin stained sections. Inducible nitric oxide synthase (iNOS), bone morphogenetic protein-4, the cluster of differentiation (CD)-68, cysteine-aspartic proteases-3 (Caspase 3), and B-cell lymphoma-2 (Bcl-2) expressions were evaluated via immunohistochemistry. Periodontitis group had highest alveolar bone loss, and these levels significantly decreased in LDO and HDO groups. Both 12 and 24 mg/kg oleuropein groups significantly increased osteoblast cell counts and decreased TRAP-positive osteoclast and inflammatory cell counts. BMP-4 and bcl-2 expressions were elevated in oleuropein groups while caspase-3 expressions decreased. iNOS and CD68 were higher in periodontitis group compared to control group, but there was no significant difference between other groups. Oleuropein successfully decreased alveolar bone loss as a result of decreased osteoclastic activity, inflammation, and apoptosis and increased osteoblastic activity. |
| Anahtar Kelimeler |
| BMP-4 | CD-68 | TRAP | alveolar bone | experimental periodontitis | oleuropein |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Yöksis | 19 |
| PubMed | 17 |