Peptidase activity assays using protein substrates, (Current Protocols in Food Analytical Chemistry)
Yazarlar (2)
Prof. Dr. Özlem AKPINAR Gaziosmanpaşa Üniversitesi, Türkiye
Mıchael H Penner
| Bölüm Adı | Peptidase activity assays using protein substrates, | ||
| Kitap Adı | Current Protocols in Food Analytical Chemistry | ||
| Bölüm Sayfaları | - | ||
| Kitap Türü | Kitap Bölümü | ||
| Kitap Alt Türü | Alanında uluslararası yayınlanan kitap bölümü | ||
| Kitap Niteliği | Diğer uluslararası bilimsel kitap | ||
| Kitap Dili | İngilizce | Basım Tarihi | 01-2002 |
| DOI Numarası | – | ISBN | |
| Basıldığı Ülke | Basıldığı Şehir | ||
| UAK Araştırma Alanları |
Gıda Kimyası
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| Özet |
| The International Union of Biochemistry and Molecular Biology recommends that the term “peptidase” be used synonymously with the term “peptide hydrolase”(IUBMB, 1992). Thus, in this unit the term peptidase is used in reference to any enzyme that catalyzes the hydrolysis of peptide bonds, without distinguishing between exo-and endopeptidase activities. Peptidases may be assayed using native or modified proteins, peptides, or synthetic substrates. In this unit, the focus is on assays based on the hydrolysis of common, commercially available, protein substrates. Thus, the assays are not intended to be selective for a given peptidase; they are designed to provide estimates of overall peptidase activity. Other units in this publication focus on synthetic or “model” substrates, which can be designed for the measurement of specific endo-and/or exopeptidase activities. |
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